http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2522801-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fbaeff2750f02c52addec74237f76737 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18fc29b7521e19d5db739d181fa84cec |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2012-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a4c6e57b55bf43c17bb6b1018f13c87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e96c488b17c631cc4dcb70ac0afae691 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8159e5d338e87cec076118921148f76b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b79d41b205e2a032bcdedec334aa8583 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b6396ff5919005e897e39af5edf7699 |
| publicationDate | 2014-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2522801-C2 |
| titleOfInvention | Method for detecting transcription level of gene coding human chemokine ccl2 (mcp-1) and kit for implementing it |
| abstract | FIELD: medicine. n SUBSTANCE: group of inventions relates to biotechnology. A method for detecting an expression level of MCP1 (CCL2) gene provides assessing its iRNA amount by a polymerase chain reaction with reverse transcription (RT-PCR) with real-time signal detection in human sampled cells, tissues and biological fluids. What is developed is a kit for quantitation of iRNA of MCP1 involving combinations of oligonucleotide primers and fluorescent probes for real-time RT-PCR to detect an amount of cDNA of MCP1 genes and RPL32, ACTB, PII normalisation genes. n EFFECT: using the group of inventions enables reducing calculation errors introduced when using one normalisation gene without increasing the number of amplifications, as well as reducing calculation errors raised in a simplified suggestion of the efficacy balance of the cDNA amplification reactions of MCP1 and a normalisation gene observed in ∆∆Ct method. n 2 cl, 2 dwg, 3 tbl, 1 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2750940-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2587540-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2731062-C1 |
| priorityDate | 2012-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 425.