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grantDate 2014-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2014-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2515385-C1
titleOfInvention Method of microclonal reproduction of black alder in vitro
abstract FIELD: biotechnologies. n SUBSTANCE: in the method they cultivate callus cultures from sterile explants of stem segments, leaves, leaf stakes. Basic nutrient media are used, such as Murasige-Skuga and Woody Plant Medium with content and ratio of growth regulators: 6-benzylaminopurine - 0.2-1.5 mg/l, α-naphthyl acetic acid - 0.2-0.5 mg/l, indolebutyric acid - 2 mg/l. Further adaptation is carried out with light of 2000 lux and nutrient mode of regenerants in a greenhouse with production of planting material for forest cultures. n EFFECT: method makes it possible to produce planting material with high inherited properties. n 2 tbl
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