http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2509805-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_23022bd76b3f475342ce6ca765eab3b0 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2012-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98c246cd4fc87f33505cb1dd2ab00c98 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3345c25ee973801ebcd780131c23c22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6f403624458b7a1149571835b36b80a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cb83c45543fe4bdb5b19a10e8c4d52b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ce8406ba24261bfcf5a0a3c12a5fd48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0855813ff36138db267acb8609e7129 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ac82b2f53c7af0ae80f4ca3182f2cf0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a8a1ba5ccffc20451d41844b4c7730b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5798fa5bc1971d6431672be94fbe0ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5c5438d5c1cf1ac30da8b5b958bd1f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1d1147b4be5867d4fc4691f8d7b09b6 |
| publicationDate | 2014-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2509805-C2 |
| titleOfInvention | METHOD OF OBTAINING RECOMBINANT ANTIGEN G2 OF DOBRAVA HANTAVIRUS IN E.coli CELLS |
| abstract | FIELD: chemistry. n SUBSTANCE: invention relates to biotechnology and a method of obtaining a recombinant antigen G2 of hantaviruses. The disclosed method is characterised by that the DNA structure pGHF, which encodes a fused protein of three parts, where N-terminal position is occupied by a green fluorescent protein GFP, central position is occupied by a peptide of 73 amino acid residues with the amino acid sequence SRKKCNFATTPICEYDGNMVSGYKKVMATIDSFQAFNTSYIHYTDEQIEW KDPDGMLKDHLNILVTKDIDFDT, and the C-terminal position is occupied by a mini-domain Foldon of coliphage fibritin JS98C3 (dwg 2), is introduced into E. coli cells; cells transformed by this structure are cultured, the biomass is lysed, the insoluble fraction of the lysate is separated by centrifuging, the product of expression in the form of inclusion bodies is solubilised with methylated spirit, chromatography is carried out and the obtained product is used to detect specific antibodies in serum of patients with hemorrhagic fever with renal syndrome. n EFFECT: disclosed solution improves repeatability and sensitivity of immunoenzymatic assay when diagnosing hemorrhagic fever with renal syndrome. n 7 dwg |
| priorityDate | 2012-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.