http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2504583-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55d1c7c096067d73b42056caf8b25c4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 2012-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6f06ce3b272e2a9364287239de293f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5dd8a294d60df993b6011341dbec64c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27e9d436901f58ead09f475b2f7366fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4681a09469b4b7dcb52eb7e63a254ab8 |
| publicationDate | 2014-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2504583-C1 |
| titleOfInvention | PLASMID 40Gal DETERMINING SYNTHESIS OF α-GALACTOSIDASE α-PsGal, STRAIN Ecoli Rosetta(DE3)/40Gal - PRODUCER OF CHIMERIC PROTEIN CONTAINING AMINO-ACID SEQUENCE α-PsGal, AND METHOD FOR ITS PRODUCTION |
| abstract | FIELD: biotechnologies. n SUBSTANCE: invention represents plasmide determining synthesis of α-galactosidase α-PsGal, which includes Ncol/Sall - fragment of plasmid pET-40b(+) (Novagen) and DNA fragment, with the size of 2142 pairs of bases, which contains a chimeric gen consisting of structural part of gene α-PsGal, which is adapted on N-end for expression in E. coli cells, and a nucleotide coding a specific sequence for enteropeptidase. The invention also refers to strain E. coli Rosetta (DE3) transformed with the above plasmid - producer of chimeric protein containing amino-acid sequence of recombinant α-galactosidase α-PsGal and sequence specific for enteropeptidase. The invention also refers to a method for obtaining recombinant α-galactosidase α-PsGal, which involves the following stages: incubation of the above strain-producer in liquid nutritional medium LB during 12 hours at 16°C, deposition of bacterial cells by centrifugation, disintegration of a suspension of cells in a buffer, centrifugation of an extract, chromatography of above-deposit liquid on a column with metal affine resin, elution of protein, concentration of active fractions by means of ion-exchange resin, incubation with enteropeptidase and separation of a target product by gel-filtration. n EFFECT: invention allows obtaining more active and stable recombinant alpha-galactosidase with high efficiency degree. n 3 cl, 1 dwg, 3 ex |
| priorityDate | 2012-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.