http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2501861-C1

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filingDate 2012-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa74cd1defe354530b646abae30e9b3e
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publicationDate 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2501861-C1
titleOfInvention METHOD FOR DETERMINING ADHESIVE PROPERTIES OF BACTERIA OF ENTEROCOCCUS FAMILY BY MEANS OF CaCo-2 CELL LINE
abstract FIELD: biotechnologies. n SUBSTANCE: method involves preparation of bacterial suspension, separation of the obtained biomass of bacteria by centrifugation, dilution of the obtained biomass in physiological solution, preparation of monolayer of CaCo-2 cells, introduction of bacterial culture, cultivation of cells, flushing with physical solution, removal of monolayer with bacterial cells and counting of the amount of bacterial cells related to 1000 cells of CaCo-2; with that, bacteria refer to highly-adhesive ones if the number of bonded cells is 1010 to 3000, to average-adhesive ones of 210 to 1000, and to low-adhesive ones of 0 to 200. n EFFECT: improvement of the method.
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