| abstract |
FIELD: biotechnologies. n SUBSTANCE: antagonistic antibody is proposed, which specifically binds with a conformational epitope from a fragment 1378-1640 of an amino acid sequence Notch3, given in the description, and inhibits activation of Notch3. The specified antibody is characterised by the fact that it contains variable areas of light and heavy chain, with a set of appropriate CDR1-CDR3, amino acid sequences of which are given in the description. An antibody may additionally contain a mark. The following is proposed: versions of a coding nucleic acid, an expressing vector, a cell for antibody production, containing the specified vector. The method is described to produce antibodies by means of cell cultivation. Versions of antibodies application are disclosed: to detect a disease related to Notch3, for production of a medicinal agent for treatment of an individual with a disorder or a disease related to Notch3, for treatment of Notch3-related disease or disorder. n EFFECT: using the invention may find application in medicine in treatment or prevention of Notch3-related diseases or disorders. n 25 cl, 18 dwg, 4 tbl, 12 ex |