http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2489440-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55d1c7c096067d73b42056caf8b25c4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
| filingDate | 2012-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76e604bc64eae65b61f5ad081319f9df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fcf0170ae1ec0b297c5965bd1d7025b |
| publicationDate | 2013-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2489440-C1 |
| titleOfInvention | Method to produce estrogen-binding protein associated with malignant tumors |
| abstract | FIELD: biotechnologies.SUBSTANCE: residue A(waste produced during industrial production of a gamma-globulin fraction from abortive blood at the first stage of fractioning) is extracted by 0.05 M sodium-acetate buffer. The produced extract is centrifuged to produce a supernatant. The produced supernatant is chromatographed, and rechromatography is carried out on DEAE cellulose. At the same time the ballast proteins are eluated with 0.05 M sodium-acetate buffer with the following eluation of ESB-containing fraction with a linear gradient of molarity of sodium-acetate buffer from 0.05 M to 0.25 M. Collection of fractions eluated in the range of 0.075 M - 0.15 M of sodium acetate with further concentration, dialysis against distilled water and lyophilisation. The dried fraction is dissolved in 0.015 M sodium-acetate buffer and applied onto KM-cellulose balanced with the same buffer (0.015 M sodium-acetate buffer) with the following performance of the stepped eluation, concentration of the fraction, containing ESB and a-1-acid glycoprotein (?- 1- AGP) and produced with eluation of 0.15 M sodium-acetate buffer. Concentration of this fraction. Dialysis against 0.01 M tris HCl-buffer containing 0.15 M NaCl with subsequent application of the produced fraction onto a column with an immunosorbent IgG anti - ?- 1- AGP-sepharose. At the same time the fraction, which is not bound with immunosorbent, is dialysed and lyophilised with subsequent performance of gel-penetrant HPLC on the column with T8K-gel in 0.1 M potassium-phosphate buffer, containing 0.1 M potassium chloride and 5 mM of trilon B. The fraction containing highly pure ESB is dialysed against distilled water and lyophilised.EFFECT: invention makes it possible to expand arsenal of facilities used in diagnostics of malignant tumors.1 tbl, 1 ex |
| priorityDate | 2012-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.