http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2486524-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ba1df94bceb73f563eb53f592a0d8229 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-563 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2011-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f4a69c1a8f2d7b6ee2606e1ff5b57ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a60a1ea67855ccff73250cab766be99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a3b761706753c00322ce8d829f9e075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7862f4f26fc2030c0ed2a32caa554296 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9cbee36bf2ef3f69c081aa62a00adc2 |
| publicationDate | 2013-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2486524-C1 |
| titleOfInvention | Method for determining anthrax lethal factor proceeding from immunodetection associated with polymerase chain reaction |
| abstract | FIELD: medicine. n SUBSTANCE: method under the invention involves determining an anthrax lethal factor (LF) by PCR-associated immunodetection. The LF protein is adsorbed on microplates or microstrips with using a pre-adsorbed specific monoclonal antibody (MCAB). Further, the immobilised LF protein is bound by the biotylated MCAB recognising another epitope of the LF protein. Thereafter, a complex of the LF protein and the biotylated antibody is detected using a noncovalent conjugate of DNA fragments with neutravidin serving as a matrix for PCR amplification with real-time fluorescence signal detection. Fluorescence levels are recorded. Changing the fluorescence levels in the respective plate wells or strip tubes as compared to the reference, the presence of the lethal factor in the analysed samples is stated. n EFFECT: method for determining the anthrax lethal factor is characterised by high sensitivity and specificity, allowing diagnosing the lethal factor protein accumulation at the early stages of anthrax infection, beginning an antibiotic therapy of an infected patient in proper time. n 5 dwg, 5 ex |
| priorityDate | 2011-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.