http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2486247-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0a9e6421e9257148abad101e1ce6c162 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57426 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2007-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7511c40600eec110ec657332208f2805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50f927165e27857fb3da3a28360e0569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f1f99749b973d3deb5d8fbe03b1a721 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe64ac1a37ebfb7243ad9696fc3744c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f04578f381e3db0ee237f77215a4507f |
| publicationDate | 2013-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2486247-C2 |
| titleOfInvention | Method of identification agent based on high throughput screening |
| abstract | FIELD: biotechnology. n SUBSTANCE: method comprises the following stages: preparing cells in the first set comprising at least 96 receivers, adding an agent at least to one receiver; incubating the agent with the cells, lysing the cells by adding a buffer which is hypotonic or comprises detergent, and contains an inhibitor of DNAase; removal of RNA from the cell lysate with ribonuclease; centrifugation of the first set of receivers at low speed; transfer of a supernatant containing DNA fragments to the second set of receivers; adding a detectable compound capable of intercalation with DNA fragments in a buffer for detection containing DNAase inhibitor to at least one receiver; measuring the amount of intercalated detectable compound; and comparing the amount of intercalated detectable compound to the control for determining the difference that enables to identify the said agent as a modifying agent if the difference exceeds a predetermined limit. n EFFECT: invention enables to detect agents that affect the formation of DNA fragments in cells using high performance screening applications. n 10 cl, 14 dwg, 2 tbl, 11 ex |
| priorityDate | 2006-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.