http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2485177-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e1c8f52d19c19e2a0bde5443644125c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1cba72b64314187f4854835431509ded |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bca0f853543945d2d35ad3b2fb98d58b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd391d4fca12e29d9e51d3ed63a58ed2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bede658c14efaefcfb93d4bba5babfc8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9aa3a56f17810a4a3528b343e80968e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60ea9a559592e92ce3156a19316330a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db360a5f22882111318a946fae8832b2 |
| publicationDate | 2013-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2485177-C1 |
| titleOfInvention | METHOD OF DETECTING RIFAMPICIN-RESISTANT ISOLATES Mycobacterium tuberculosis |
| abstract | FIELD: biotechnology. n SUBSTANCE: method of detecting the mutations in rpoB gene by carrying out PCR is proposed, when in "real-time" the fragment of rpoB gene is created using primers Rpo11 (5'-GACGTCGAGGCGATCACAC-3') and Rpo12 (5'-GGCACGCTCACTTGACAGAC-3'), after that HRM-analysis is carried out. To increase the reliability of HRM-analysis of mutations in rpoB gene the equal amounts of tested DNA and DNA H37Rv are mixed. The concentration equalisation is carried out using quantitative PCR with use of the same pair of primers with creation of a calibration curve and use of regression equation. n EFFECT: method due to its high specificity and sensitivity enables to identify reliably and quickly the type of mutations in the rpoB gene associated with the emergence of resistance to rifampicin. n 2 dwg |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2619258-C2 |
| priorityDate | 2012-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.