http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2480746-C1

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15
filingDate 2011-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c35bc6ff314ac29200d4e11f13e09817
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4e0adc443e6114a84ead94f688207cc
publicationDate 2013-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2480746-C1
titleOfInvention Method for polysaccharide analysis in herb of violet species
abstract FIELD: medicine. n SUBSTANCE: method for polysaccharide analysis in herbs of Violet species involving grinding of raw materials, water extraction of a polysaccharide complex when heated, addition of an agent, filtration and polysaccharide quantification by formula, differs by the fact that the raw material is Viola uniflora herb; water extraction lasts for 1 hour in ratio of the raw material : the extractant as 1:50; the extract is filtered, and the filtrate is added with 8% hydrochloric acid in ratio 1:1; the mixture is kept for 2 hours on a boiling water bath, then cooled and reduced to pH=6.5-7.0; thereafter the mixture is added with 1% picric acid and 20% sodium carbonate in ratio 1:3; the prepared mixture is kept on a water bath for 30 minutes, then filtered; it is followed by spectrophotometric analysis of the filtrate in a tray of the absorption layer thickness of 1 am at wave length λ=460±2 nm, while polysaccharides are quantified at a glucose basis by formula. n EFFECT: method enables assessing the quality of medical plants by quantifying accurately polysaccharides contained in herbs of Violet species. n 2 tbl, 1 ex
priorityDate 2011-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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