| abstract |
FIELD: medicine. n SUBSTANCE: what is presented is a method for preparing the substance L-lysine-alpha-oxydase (LO) with the use of the Trichoderma cf. aureoviride Rifai BKMF-4268D producer strain. It is followed by fermentation on a medium containing nitrogen, phosphate sources and wheat bran. It is followed by recovery and purification of the enzyme in the following sequence of the procedures: culture fluid dialysis, adsorbent treatment, impurity sedimentation in 20%-saturated ammonium, hydrophobic and ion-exchange chromatography. It is followed by preparing L-lysine-alpha-oxydase of molecular weight 115-116 kDa, isoelectric point 4.25, optimum pH - 7.4. n EFFECT: higher yield of LO. n 5 tbl, 5 ex |