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filingDate 2011-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2012-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d1f0d598156ca484a441d0ff7b92aaa
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publicationDate 2012-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2460802-C1
titleOfInvention Method for identifying yersinia enterocolitica
abstract FIELD: medicine. n SUBSTANCE: identifying a yersiniosis agent is enabled by using the intestinal yersinia pague FK-100 stripped on a double-layer grass lower layer of which represents an agar medium (pH 7.1) with added ampicillin in the concentration of 50 mcg/ml, while an upper layer of which is produced of the strain being tested cultivated on 1.5% Hottinger's agar at 28°C during one day and inoculated in 4 ml of Hottinger's broth, incubated at temperature 28°C to the final concentration n·10 8 m.c./ml; thereafter 0.3 ml of the prepared culture is mixed with 4.5 ml of 0.7% Hottinger's agar melted and cooled to 45°C with 50 mcg/ml of ampicillin and poured as a second layer on the agar plates. The inoculation is incubated at 28°C for 20-24 hours. Yersinia enterocolitica in the sample being tested is identified from the other types of yiersinia by the presence of phagolysis on the growth culture. n EFFECT: method provides complete identification effectiveness. n 1 tbl, 3 ex
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