http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2458635-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd0f87e661b4360800b6e8a2fd26b458 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61B17-00 |
| filingDate | 2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa872cbabfd7e3b9e15e4154f88cf7c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e856b683a7f5bbbe80779f2d0131ce5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40004b70b8d9f35f30652a78e26e7e64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a9d0360d9b5b081ab549cc3387a1e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad77f7f306997f24de2a529d36846ee2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0cbdac355f4279736a8491dbfcf549e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef898c78e85bc60aac127c00a0457d7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03df358d85063dd2e0af45ccb0f434c0 |
| publicationDate | 2012-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2458635-C1 |
| titleOfInvention | Method of obtaining tracheobronchial bioimplant |
| abstract | FIELD: medicine. n SUBSTANCE: invention relates to medicine and veterinary, in particular, to transplantology. In order to obtain bioimplant mechanical cleaning of donor's tissue of trachea/bronchi from connective and adipose tissues is carried out. After that donor's tissues of trachea/bronchi are washed with distilled water and incubated for 10-14 days in 5-10% solution of chlorine-containing oxidant. After that tissues of trachea/bronchi are washed with physiological solution and cryoconservation (in case of necessity) is performed. Mesenchymal stem cells CD271+ are isolated from recipient's bone marrow by method of immunomagnetic separation and their generation is carried out. Suspension of recipient's epithelial cells is obtained. Matrix of trachea/bronchi is covered with protein of recipient's blood serum and surface of bioimplant is inoculated with epithelial and mesenchymal cells. Bioimplant is incubated in RPMI-1640 medium until implantation. n EFFECT: method ensures short cycle of preparation (15-21 days) of individualised bioimplant based on sterile matrix with preservation of framework function of organ, covered with protein-cellular envelope, which do not possess heterogenic immune properties. n 25 dwg, 4 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2792542-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2499611-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103255104-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103255104-A |
| priorityDate | 2011-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.