| abstract |
FIELD: medicine. n SUBSTANCE: invention refers to nucleic acid constructs used to produce recombinant parvoviral vectors in insect cells containing such constructs, and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferentially contains a first nucleotide sequence coding parvoviral Rep proteins wherein a translational initiation codon of the parvoviral Rep78 protein represents a suboptimal initiation codon which have an action of a partial exon skipping in expression in the insect cells. The insect cell additionally contains a second nucleotide sequence containing the nucleotide sequence with at least one parvoviral (AVV) inverse terminal redundancy (ITR), and a third nucleotide sequence containing sequences coding core parvoviral proteins. n EFFECT: method improvement. n 30 cl, 6 dwg, 2 tbl, 3 ex |