http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2455015-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_75cf4d02cc8cbcf236e220a1d604bf92 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-16 |
| filingDate | 2011-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e5a380d6b31de87fd90679907a13c4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48f49ee34267f749777fc1d53fbdab68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d138ef7f0f49237d1f060ead9264f35e |
| publicationDate | 2012-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2455015-C1 |
| titleOfInvention | Method for preparing blood serum of cattle foetuses for animal and human cell culture |
| abstract | FIELD: medicine. n SUBSTANCE: invention refers to biotechnology, virology and immunology, and is applicable in animal and human cell line culture. The method involves blood sampling, serum separation and two-fold filtration sterilisation. The foetus blood is sampled with using a sterile system and placed in glass bottles 250 ml containing hypertonic saline. The bottles are filled 80-100%. The blood is sampled from a recess at a cranial parts of a sternum. The serum is separated by centrifugation at 2000 rpm for 30-40 minutes. After the sampling, the serum is frozen at -15…-20°C for at least 7 days. After the defrosting at +4…+20°C, the serum is collected, and strongly laked layers are to be removed. Thereafter the prepared serum is pre-filtered thought the depth filters EKS, and then - thought the sterilising membrane filters at +2…+6°C. n EFFECT: method allows higher end product yield and improved quality. n 7 tbl, 7 ex |
| priorityDate | 2011-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID5234 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID448670727 |
Total number of triples: 15.