http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2447143-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_674c86d5bce59c42199568e574add85f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_67881edfb16851f837166869113dcc02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f084275e86270e3c63b6b5ef21057031 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2008-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04171286bbefe32f6d8c9073d643b402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c4ee34062542fcbe248de9e2291173c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30a4b75826d7a9a2f8b4c31e921ef9d0 |
| publicationDate | 2012-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2447143-C2 |
| titleOfInvention | METHOD FOR SUBMERGED CULTIVATION OF Bacillus brevis FOR PRODUCING GRAMICIDIN S |
| abstract | FIELD: chemistry. n SUBSTANCE: disclosed is a method of cultivating Bacillus brevis strain 101 for producing gramicidin S. Submerged cultivation of a culture is carried out on a synthetic culture medium. The medium contains yeast autolysate and casein hydrolysate in concentration of 0.1 g/l and 0.2 g/l on amine nitrogen, glycerine in concentration of 20 ml/l, edible 40% lactic acid 2.0-4.0 ml/l, ammonium phosphate-chloride 0-3.4 g/l, di-substituted ammonium phosphate 0.85-4.5 g/l, mono-substituted potassium phosphate 0-1.0 g/l, magnesium sulphate heptahydrate 0.9 g/l, sodium citrate 1.0 g/l. Content of amine nitrogen in the initial medium is equal to 1.3-1.6 g/l. When concentration of amine nitrogen falls to less than 1.4 g/l, a concentrated culture solution is added to the medium until achieving concentration of 1.75 g/l. The concentrated culture solution contains glycerine, edible 40% lactic acid, di-substituted ammonium phosphate and chloride and magnesium sulphate with ratio of concentration of glycerin, lactic acid, nitrogen, phosphorus and magnesium equal to 1:(0.008-0.032):(0.027-0.036):(0-0.008):(0.002-0.008). During growth, the rate of stirring is increased from 200 to 500 rpm. pH is kept at 6.5-6.8 by adding potassium and sodium hydroxide. The process is stopped 2-6 after the onset of a stationary phase. n EFFECT: method enables reproducible production of a large amount of gramicidin S. n 10 tbl, 5 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-030630-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017209651-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3660141-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2627182-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2743396-C1 |
| priorityDate | 2008-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.