http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2441916-C1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e2cc0acca3c2f919f58754e780efc45a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb1085939f1e15fb8ea36f5d81436dcc |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
filingDate | 2010-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2012-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95dd56d67470e46d8eb31f605ee879fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cb9aba336dca1d5d87524d040c8cf25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f8aff9a750b3cadb93b1250e864e793 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7fc2339ddfa04e78ac64e39dc58b098 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0cf02b38901994f47c1fc287bac8b1e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a09b089935fc1e6553b3a36bc38ebab3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13f83298df9a950449ed93957a76db46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c076799c3db0c77845123d734397ff6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f49365aa34657891ba8d8f578c0c498d |
publicationDate | 2012-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2441916-C1 |
titleOfInvention | RECOMBINANT PLASMID DNA pACYC-LANS(KM), STRAINS OF Escherichia coli BL21(DE3), TRANSFORMED RECOMBINANT DNA pACYC-LANS(KM) AND METHOD FOR PRODUCING A SUBSTANCE OF THE RECOMBINANT L-ASPARAGINASE FROM Erwinia carotovora |
abstract | FIELD: biotechnology. ^ SUBSTANCE: invention relates to the area of biotechnology and genetic engineering. A recombinant plasmid DNA pACYC-LANS(KM) was constructed to be expressed in the cells of Escherichia coli of the polypeptide of recombinant L-asparaginase of Erwinia carotovora (rec-ASP-ECAR), was proposed producer strain rec-ASP-ECAR that is obtained from transformation of competent cells of Escherichia coli BL21(DE3) using the constructed recombinant plasmid DNA pACYC-LANS(KM), a method for growing the strain with its splitting from the obtained biomass and purification of recombinant L-asparaginase from Erwinia carotovora was developed. ^ EFFECT: enhanced biosynthesis of rec-ASP-ECAR polypeptide and high output and purity of the target product which is achieved by the implementation of a simple method for producing recombinant asparaginase. ^ 4 cl, 4 dwg, 2 tbl, 5 ex |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2562166-C1 |
priorityDate | 2010-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 230.