http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2440417-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3b1e4ec6f34b8826473bf490cce410cb |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2011-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ffed724dc9a1b3ed515e59e0284d6d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_692525fa55b87257e405c5926c0f67e9 |
| publicationDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2440417-C1 |
| titleOfInvention | Method of purification of recombinant granulocyte colony-stimulating human factor |
| abstract | FIELD: medicine. n SUBSTANCE: described is method of purification of recombinant granulocyte colony-stimulating factor (filgrastim) of human. Method characteristics: at the stage of purification from coarse pollutions applied is sorbent SP-Sepharose Fast Flow, elution is carried out by gradient 0.5 M NaCl in acetate buffer pH 5-5.5, stage of fine purification is carried out by ion-changeable chromatography on sorbent YMC BioPro S30, at the stage of purified product concentration applied is sorbent CM-Sepharose Fast Flow, and elution is carried out by gradient 0.5 M NaCl in acetate buffer pH 5-5.5, additionally carried out is stage of gel-filtration by sorbent Sephadex G25, elution is carried out by 13.3 mM sodium-acetate buffer, pH 3.9. n EFFECT: invention makes it possible to considerably reduce waste of protein during purification. n 2 tbl, 2 ex |
| priorityDate | 2011-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.