| abstract |
FIELD: medicine. ^ SUBSTANCE: patient's blood serum is processed for 1 h in darkness at 40C with Sudan B dye solution, agarose solution is added, heated to 55C mixture is introduced into hollow in agarose gel with volume 4ù20ù10 mm, electrophoregram is fixed, dried, densitometry is carried out, to 0.3 ml of patient's blood serum sample additionally added is 0.1 ml of 0.1% triton X-100 solution and incubated for 15 min at 20C, mixture is mixed by method of shaking 120 times per 1 min, and disintegration of lipoproteins is carried out. ^ EFFECT: method application makes it possible to determine maximally possible intensive minor fractions of blood LP, which increases its sensitivity and accuracy. ^ 3 dwg, 1 ex |