http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2410441-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d26998ae0985ac95af35c7d162c8d5e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc5388f29adfbbb6c736ae8e486e7e92 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-155 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-708 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2007-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3e2486d4c21821f53d45695be89936e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_435ecba72f6bfb6b463c42896b808072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ead3ab2eb1753d7d4bb6c4e3b05a8d81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73c3ad67bb4f6c326c97479cc3b5b38d |
| publicationDate | 2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2410441-C2 |
| titleOfInvention | Set and method for detecting human papilloma virus using set of oligonucleotide granules |
| abstract | FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention relates to biochemistry, particularly to a set of primers for amplifying the L1 gene of the human papilloma virus (HPV), a set for detecting the human papilloma virus (HPV) genotype and methods of detecting human papilloma virus (HPV) genotypes. The method involves primary PCR amplification of the HPV L1 gene in an analysed sample using a set of direct and reverse primers which are specific for the L1 gene section. Further, secondary PCR amplification of the product of primary PCR amplification of the L1 gene is carried out using a reverse primer to obtain a biotin-labelled single-stranded L1 gene. The product of secondary PCR amplification and biotin-labelled single-stranded L1 gene undergo a hybridisation reaction with one or more probes for detecting the HPV genotype or a probe for detecting the HPV genotype. Further, the product of the hybridisation reaction reacts with phycoerythrin, bonded with streptavidin. The level of fluorescent substance is measured and probes are identified in the product of the hybridisation reaction to identify the HPV genotype. Further, the HPV genotype is determined, as well as the level of its presence in accordance with probes and the level of the fluorescent substance. ^ EFFECT: invention provides a method for detecting HPV in a sample with high sensitivity, sufficient for detecting extremely small quantities of HPV in the sample. ^ 8 cl, 2 dwg, 1 tbl, 4 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2556808-C2 |
| priorityDate | 2006-03-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 186.