http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2410395-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_980dbd876383dd78c814e04a57b1cde3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 |
| filingDate | 2008-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d1659d74fc941432cd65642ed53f799 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b080cdfb4af6bb3d740297b0289280be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79e1af8d435d1a10d32859b99f2f622b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0dfdf1077d26bb16eebdc9cd1c134871 |
| publicationDate | 2011-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2410395-C2 |
| titleOfInvention | Method of producing monospecific antibodies to plant cell proteins |
| abstract | FIELD: chemistry; biochemistry. n SUBSTANCE: invention relates to biotechnology and is meant for producing monospecific antibodies to plant cell proteins. Purified protein antigens are produced via chromatography and electrophoresis in polyacrylamide gel. The antigen used is a marker protein of meristematic cell of wheat. Before immunisation, the obtained protein antigens are incubated in tris-HCl with a buffer solution containing sodium dodecylsulphate and 2-mercaptoethanol for 12-24 hours at 2-10°C. Laboratory animals are immunised three times with two-week breaks. The first immunisation is done in popliteal lymph nodes with a portion of the crushed gel containing the antigen with Freund's complete adjuvant, and the second and third immunisations are done in peritoneum active sites with a portion of the crushed gel with incomplete Freund's adjuvant. Monospecific serum, which contains the antibody to the investigated antigens, is obtained from the blood of the immunised animals. The antibodies are purified by fractionating antiserum with caprylic acid followed by desalination and cation-exchange chromatography. n EFFECT: invention increases specificity and titre of obtained antibodies owing to additional purification operations. n 6 cl, 5 dwg |
| priorityDate | 2008-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.