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publicationDate 2010-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber RU-2406090-C2
titleOfInvention Method of immunochromatographic assay of milk and dairy products for antibiotics
abstract FIELD: medicine. n SUBSTANCE: there is used a multi-membrane composite in certain regions of which there are applied: 1) coloured marker particles in complex with antibiotic specific antibodies 1, 2) antibiotic conjugate with a carrier molecule providing conjugate immobilisation on the membrane, 3) membrane immobilised antibodies 1 specific antibodies 2 (a reference zone). Once the multi-membrane composite contacts with sampled milk or dairy product to be tested, a coloured strip or strips are formed in a certain zone or zones of the multi-membrane composite to be interpreted as follows: a) the reference zone coloured - the presence of the antibiotics to be detected in the sample, b) the analytical and reference zones coloured - the absence of the antibiotics to be detected in the sample. A characteristic property of the offered approach is the use of a specially specified combination of antibodies 1 of high reaction rate with an antigen at a kinetic association reaction constant not less than 10 5 M -1 sec -1 , the membrane carriers of pore size not less than 10 mcm and coloured marker particles of average diametre not less than 30 nm. n EFFECT: invention allows non-laboratory express assay of dairy products for antibiotics. n 4 dwg, 4 ex
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