http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2381273-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_82cc33723ce303553b6a42e3fb95dab4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_446f13cb64b77b7311207ab9b07aa6fd |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P37-00 |
| filingDate | 2008-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48b8f0f515a75c4fab0e5f9acbbb2b0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9df058525f9728f30030e6dd18f66999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d14c5ce015c44508d5ad235c08eeef30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfe10dd64ef3ed1ad3d0d47aedf6c1be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e5b683837d67f0e6352f381026632ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12953b706e93ff4ad229dc5d4cf54149 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc86c20006fbc87f9a6722fe1d81d600 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dac70dd1ba4521e3a95c660adcc5ef1 |
| publicationDate | 2010-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2381273-C2 |
| titleOfInvention | Method for production of heterogeneous biocatalyst, biocatalyst based on hydrolase of alpha-aminoacid ethers and method for synthesis of aminobeta-lactam antibiotic under action of this biocatalyst |
| abstract | FIELD: biotechnologies. n SUBSTANCE: method for production of heterogeneous biocatalyst for fermentative synthesis of aminobeta-lactam antibiotic based on intracellular hydrolase of alpha-aminoacid ethers from bacteria of Xanthomonas type, including damage of biomass in cells of Xanthomonas rubrilineans, extraction of ferment from it into water phase and its further immobilisation. Besides, damage of cells is carried out in stages, first damaging their integrity by cryogenic action, and then by treatment of water suspension of thawed cells by butyl acetate. Extraction of ferment into water phase is carried out in process of treated cell suspension heating first in conditions of spontaneously established gradient of pH, and then at constant value of pH. Produced cell-free extract is used for procedure of ferment immobilisation by means of its deposition by polyethylene glycol in the form of ferment aggregates and their further cross linkage in presence of glutaric dialdehyde. Thus obtained heterogeneous biocatalyst contains up to 0.95 g of protein/g of dry biocatalyst and has synthetase activity of up to 2200 ME/g of dry biocatalyst. n EFFECT: invention makes it possible to improve efficiency of biocatalyst production procedure, to increase activeness and stability of produced biocatalyst. n 6 cl, 2 dwg, 4 tbl, 9 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2650668-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2535893-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2499830-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2502797-C1 |
| priorityDate | 2008-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.