http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2379685-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2c9cd9998822b37f1c44f5a63eaa1da5 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 2007-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff03d93f6681f2e51ad1f70ae8745c11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_876e9357498a19bc748601773269f060 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68051a2f559e83b34c27a5449e8ca71b |
| publicationDate | 2010-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2379685-C2 |
| titleOfInvention | Method of carrying out express nbt-test of leukocytes in peripheral blood of dogs |
| abstract | FIELD: veterinary. n SUBSTANCE: method of carrying out express NBT-test of leukocytes in peripheral blood of dogs includes mixing of blood with dissolved nitro blue tetrazolium (NBT), incubation, microscoping and calculation of positive and negative cells. In test-tube introduced and mixed are: 0.05 ml of fresh heparinised blood, 0.05 ml of 0.2% NBT solution on phosphate-salt buffer, then said mixture is incubated during 30 minutes at T=37 degrees Celcius, after the end of incubation 0.3 ml of 3.5% acetic acid solution is introduced into test tube, thoroughly mixed; aliquot of obtained mixture is taken and used to charge Goryaev chamber, cells are microscoped under magnification 40×10, ensuring minimal visibility of non-stained cells, NBT-positive cells, which contain formazan in blood cytoplasm, and NBT-negative cells, which do not contain formazan, are calculated in differentiated way. n EFFECT: creation of simple, fast and efficient method of carrying express NBT-test in leukocytes of peripheral blood of dogs. |
| priorityDate | 2007-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.