http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2350654-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7108bb26b56eb1ae69a2421c93525b20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2004-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1144fac4024a044ca0bb84232b717587 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47e17a5367f7cc01f7e90e05623cf1f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d3e4297cdc9df3a695c9134b4d19219 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62ee4fe351dc7abc50029da7c0cd15aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_224f3fcd8a75d2cea574e41cb75a3d1b |
| publicationDate | 2009-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2350654-C2 |
| titleOfInvention | Method of mass production of multimeasured mannan-binding lectine |
| abstract | FIELD: pharmacology; biotechnology. n SUBSTANCE: invention concerns biotechnology area, in particular to the gene-engineering way providing mass production multimeasured recombinant human Mannan-Binding Lectin (MBL), also can be used in the biomedical industry. The offered way includes a) a cultivation stage of cells-owners lines CHO, transfectant with a recombinant vector pMSG-MBL, containing sequence of human MBL coding area, and b) a clearing stage of the recombinant protein. Thus at the first stage preferably use new cellular line CHO MBL D1-3, deposited with the Korean collection of sample cultures at number KCTC 10472BP which is cultivated in a protein-free medium with bioreactor use, and on the second selective clearing of high-molecular form MBL of medium cultivation is spent, providing separation of samples with the help anion exchange chromatography, their drawing on a column with an immobilised MBL-binding protein on it , in particular with glycosilated protein of a cover of a virus pre-S, in the presence of ions of calcium and the subsequent elution using a buffered solution with EDTA or EGTA. n EFFECT: high output of functionally active product opens possibilities of its application by working out of therapeutic agents for treatment of virus, bacteriemic or fungoid infections. n 6 cl, 11 dwg, 9 ex |
| priorityDate | 2004-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 402.