http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2350651-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a737ae1ccaaa9f092bb9277bca90cee |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2007-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b56f137e428c3fbe4f2828a02e2fe16d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5146a757a36b42e2a169e5d1e051c4e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44bd0f5e466b5eda02af2bb08b0cd7a2 |
| publicationDate | 2009-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2350651-C1 |
| titleOfInvention | Method of recipient selection for realisation of transduction bacillus anthracis and closely related bacilli |
| abstract | FIELD: medicine; microbiology. n SUBSTANCE: efficiency of the transducing phage crops multiplied on the strain-donor, and strains of the future recipients. Quality of the recipient is checked at statement of an intraspecific and interspecific transduction, by addition of 1 ml transducing phage of various concentration to 1 ml recipient cultures B.anthracis, B.thuringiensis and B.cereus with the various characteristic of efficiency of the transducing phage crops, and the control - 1 ml recipient culture admix about 1 ml of a phage buffer. Incubate during 20 minutes at 33°C in a shaker at 100 sh. rot./minute, then in all assays it is brought on 1 ml of a preparation of cellular receptors of corresponding recipients. Again incubate during 30 minutes at 33°C. Then in each assay bring on 1 ml of corresponding receptors and inoculate on 0.2 ml from skilled and control assay on the selective Hottinger mediums, grown Tet R colonies - transductant are calculated in 48-72 hours after incubation of crops at 35°C. The recipient is considered optimum for transduction if efficiency of the transducing phage crops is peer 0.5-1.0 (relation of an indicator of concentration transducing phage received on the strain-donor to an indicator of concentration received on the selected strain-recipient). n EFFECT: selection of optimum recipients for realisation of a transduction from an available collection of strains Banthracis and closely related bacilli. n 1 dwg, 2 tbl, 2 ex |
| priorityDate | 2007-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.