http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2347811-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ae139cf0230805736f19388d821c014 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate | 2007-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0c16844720813abbdc35548eeff43ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cee0324aa2b9b2449be63b28965025bb |
| publicationDate | 2009-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2347811-C1 |
| titleOfInvention | RECOMBINANT PLASMID DNA pET-KSI-Buf2, CODING HYBRID PROTEIN, CONTAINING ANTIMICROBIAL PEPTIDE BUFORIN-2, STRAIN OF Escherichia coli BL21(DE3)/pET-KSI-Buf2-PRODUCER OF INDICATED PROTEIN AND METHOD OF OBTAINING ANTIMICROBIAL PEPTIDE BUFORIN-2 |
| abstract | FIELD: chemistry; biochemistry. n SUBSTANCE: present invention pertains to genetic engineering, specifically to microbial production of recombinant proteins, and can be used in biotechnology, medicine and veterinary medicine. Designed is a recombinant plasmid DNA pET-KSI-Buf2 with size 4066 "п.о.", coding hybrid protein KSI-Buf2, containing antimicrobial peptide buforin-2. Through transformation of cells of the parent strain of the Escherichia coli BL21(DE3) bacteria of recombinant plasmid DNA pET-KSI-Buf2, the strain Escherichia coli BL21(DE3)/pET-KSI-Buf2 - producer of hybrid protein, is obtained, containing antimicrobial peptide buforin-2. The Escherichia coli BL21(DE3)/pET-KSI-Buf2 strain is then cultured, with subsequent cell disintegration, separation and washing of inclusion bodies, containing hybrid protein KSI-Buf2, solubilisation of the hybrid protein and its splitting using bromine cyan in an acid medium on a methionine residue, dissolving of the target peptide and cleaning it using high performance liquid chromatography method. n EFFECT: obtaining identical natural buforin-2 with high output, using simple technology. n 3 cl, 5 dwg, 1 tbl, 4 ex |
| priorityDate | 2007-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 202.