| abstract |
FIELD: agriculture. n SUBSTANCE: development of a reliable means for the identification of the corresponding transformation event in the plant genome. As a result of agrobacterial transformation of the Lugovskoy potato with a genetic maker containing the gene cryIIIa, and the analysis of the resulting transgenic plants demonstrating resistance to the Colorado beetle, the transgenic line (transformation event) 1210 amk has been selected. In the genomic DNA of the plants belonging to this line a fragment has been identified and sequenced, related to the genetic maker insertion area and being a recombinant sequence, one part of which relates to the genetic maker inserted, and the other part relates to the flanking area of the Lugovskoy potato genomic DNA. It is proposed to use the identified recombinant sequence as an identifier of this unique transformation event. n EFFECT: obtaining transgenic potato that is resistant to Colorado beetle and complies with requirements of biological and food safety on basis of high yield Russian variety. n 5 cl, 5 dwg, 7 tbl, 7 ex |