http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2324740-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a737ae1ccaaa9f092bb9277bca90cee |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 2006-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2008-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93e5c47e5c6db1081ea0a3c74c83197a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9b656c0b3aae868fea8f6ca34c22fcd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1a6559980e806c6efde3feda277a4ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6dc9e6f7c9c0a2716953cd6e672d60dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80f5fe04ba395cbfff2dc9693cc365fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca85a0d0fea8a70ff6cba29f97767f54 |
| publicationDate | 2008-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2324740-C2 |
| titleOfInvention | MODE OF ISOLATING PROTEINS OF TOXIN-COREGULATED ADHESION PILI AND OmpU OF COMMA BACILLUS OF CLASSIC BIOVAR |
| abstract | FIELD: chemistry, organic, biotechnologies. n SUBSTANCE: invention relates to biotechnology. Comma bacillus cellular mass will be obtained, the proteins will be removed from the surface with orbital rotation of the bacterial mass with the velocity of 2500 r.p.m. during 2 minutes in the vibration mixer. The cellular mass will be fractioned with low velocity centrifuging at 3-4 thousand r.p.m. The supernatant will be fractioned with high velocity centrifuging at 18-20 thousand r.p.m. Then the sediment will be selectively extracted for obtaining supernatant containing purified proteins TCAP and OmpU with the solution 125 mM of ethanolamine (pH 10.5) at 4°C in the shaker. From the supernatant, proteins of toxin-coregulated pili adhesine and OmpU will be isolated. n EFFECT: isolation of proteins of toxin-coregulated pili adhesine and OmpU from supernatant. n 3 cl, 3 dwg, 4 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2663102-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2703282-C1 |
| priorityDate | 2006-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.