| abstract |
FIELD: biotechnology, microbiology, biochemistry, enzymes. n SUBSTANCE: method involves culturing corynebacterium comprising the inserted gene encoding neutral metalloprotease from Streptoverticillium and prostructural moiety of protransglutaminase is split off by neutral metalloprotease produced by this microorganism. Features of neutral metalloprotease are given in the invention text. Also, invention relates to nucleic acid molecule encoding neutral metalloprotease. Invention provides preparing protease that can be used for producing transglutaminase that splits prostructural moiety of protransglutaminase selectively. n EFFECT: improved producing method of enzyme. n 8 cl, 12 dwg, 2 ex |