http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2315606-C2
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P27-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 |
| filingDate | 2005-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2008-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2008-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2315606-C2 |
| titleOfInvention | Method for preparing medicinal agent for treatment of myopia and eye scleral envelope diseases and vitreoretonopathy |
| abstract | FIELD: medicine, ophthalmology, preparative biochemistry. n SUBSTANCE: method involves isolation of tissue of scleral envelope central region from fresh enucleated eye of vertebrate animals wherein this tissue comprises a clibriform plate. Clibriform plate is washed out, kept in aqueous-saline physiological solution, centrifuged and supernatant is collected. Supernatant is separated by its dissolving in 100% solution of ammonium sulfate, filtered and dialyzed up to the complete removal of ammonium sulfate ions followed by separation by isoelectrofocusing method in sucrose gradient density at pH 3.5-10.0, at temperature 4-6°C and voltage 500-2000 V for 72-96 h. Then fractions of acid proteins are collected, combined and dialyzed up to the complete removal of sucrose and ampholines. Prepared protein aqueous solution is dried up to preparing a dry matter that is purified by electrophoresis method in 7.5-15% polyacrylamide gel and low-molecular fraction with value R f = 0.9-0.95 containing a regulatory peptide is collected by elution from gel with deionized water at temperature 4-7 o C for 2-5 days. Prepared fraction is dialyzed in dialysis sacs with the penetration limit value of pores 2-8 kDa at temperature 4-7 o C for 7-10 days with deionized water. After dialysis the regulatory peptide solution is dried again up to preparing flaky-like finely dispersed powder that is dissolved then in physiological aqueous-saline solution. Invention provides the enhanced percent yield of regulatory peptides possessing biological activity in superlow doses and isolation of highly purified polypeptides of low-molecular mass. n EFFECT: improved preparing method. n 6 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2585955-C1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2701566-C1 |
| priorityDate | 2005-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.