| abstract |
FIELD: biologically active compounds technology. n SUBSTANCE: invention relates to improved method for isolating individual biologically active anthocyan pigments, employed as naturally occurring pigments or biologically active components of therapeutical and cosmetic agents, from water-alcohol extract of red grape cake. Anthocyanes are sorbed on talk and then eluted with water-alcohol solution and separated chromatographically into individual anthocyans by passing solution under vacuum through column or filter filled by silica gel particles 0.040-0,063 mm in size and using, as eluent, ethyl acetate/acetic acid/water ternary mixture in volume proportions (0.67-4.67):1:1. n EFFECT: improved purification of anthocyans showing highest biological activities. n 2 cl, 1 tbl, 3 ex |