http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2298793-C1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e73b04a7e7fd17a778aa66face5e78b6 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-49 |
filingDate | 2005-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2007-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbe2ec20a5bba7216db003daddff2c6e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db54ce5a37f530a96efc51d35317d279 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_531afa58de41367fbec9c33dba6cbc89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c30396858cf91084e12481b4eca939d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4b834ae017a8bba3a542a9a3ad2494e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_62026bc911a60497edb86da396d61831 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9fb3f8cd005b7aafd8fc4dc553aeb15 |
publicationDate | 2007-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2298793-C1 |
titleOfInvention | Method for determining and separating fatty acids |
abstract | FIELD: biological engineering. n SUBSTANCE: method involves treating blood with chloroform and methanol mixture taken in ratio of 2:1, carrying out lipid hydrolysis and methylation in sulfuric acid and methanol solution at 80°C within 3 h, cooling, processing methylated sample with hexane-containing solution, drying in nitrogen flow and carrying out fatty acids gas chromatography determination and separation on polar liquid diglycol succinate phase covering surface of 50 m long column of external diameter 0.32 mm, at chromatographic oven columns temperature equal to 140°C. Column is repeatedly washed out with chloroform, methanol, acetone and hexane before introducing diglycol succinate. Diglycol succinate is distributed over internal wall of capillary column using dynamic method so that diglycol succinate layer thickness makes 0.5 mcm. Then the column is purged with nitrogen during 5-6 h 3 days long at capillary column thermostat temperature equal to 200°C. n EFFECT: increased sensitivity in determining and separating fatty acids in biological fabrics. n 2 dwg, 3 tbl |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2656132-C2 |
priorityDate | 2005-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.