| abstract |
FIELD: biotechnology, biochemistry. n SUBSTANCE: invention relates to a method for preparing human recombinant leukocyte interferon alpha-2B. Human interferon alpha-2B is prepared by culturing the transformed strain of E. coli BL21 (DES)[pAYC-ET-(hIFN-α2b)-lacI] in nutrient medium, microorganism cells rupture, removing DNA and RNA, concentrating the prepared product by washing out protein, centrifugation and denaturation of a precipitate formed in guanidine hydrochloride solution in the presence of 0.05-0.15 wt.-% of methionine followed by renaturation of interferon in the presence of 0.3-0.5 M of arginine and its purification on cation-exchange resin Soloza KG 20/30, Sp-cellulose and phenyl-Sepharose CL 4B successively. Invention provides significant increase the yield of human leukocyte interferon alpha-2B for medicinal using. Invention can be used in medicine. n EFFECT: improved preparing method. n 7 cl, 5 tbl, 3 ex |