Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-72 |
filingDate |
2004-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2006-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af71fdc8fd9bf527974fefee57d540b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a4a7fdf6b76f8f17afc1fa4e66b8564 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ee93898836872c77ff67c00452a5540 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8eeca0d82dd38dc24957f0d674726076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1ef544d331a37bd837502304f38f5a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1aba9508615ee7dab4fdd8f09dc1efcf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de4017279540210b036cc91dce22fcfa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92fe48016b2080a9b67d44fc0ac257c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7640b7d763bf16ec91ca5099976b01e |
publicationDate |
2006-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
RU-2278160-C2 |
titleOfInvention |
RECOMBINANT PROTEIN LACspCBD POSSESSING BETA-GALCTOSIDASE ACTIVITY AND ABILITY FOR SPONTANEOUS BINDING WITH CELLULOSE-CONTAINING SORBENTS, RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF RECOMBINANT PROTEIN LACspCBD, STRAIN Escherichia coli M15 [pREP4, pLACspCBD] AS PRODUCER OF RECOMBINANT PROTEIN LACspCBD, METHOD FOR PREPARING IMMOBILIZED RECOMBINANT PROTEIN LACspCBD ON CELLULOSE AND METHOD FOR ENZYMATIC CLEAVAGE OF LACTOSE |
abstract |
FIELD: genetic engineering, biotechnology. n SUBSTANCE: invention proposes a fused protein of beta-galactosidase and cellulose-binding domain of endoglucanase CelD from Anaerocellum thermophilium. The recombinant plasmid DNA pLACspCBD is constructed providing its expression in E. coli cells. The strain E. coli as a producer of indicated fused protein is obtained. A method for preparing this fused protein in immobilized form is developed that involves treatment of cellulose with hydrolyzate of the indicated strain of E. coli. Using the invention provides simplifying processing of dairy foodstuffs. Invention can be used in food processing industry. n EFFECT: valuable properties of strain and protein, improved preparing method. n 5 cl, 1 dwg, 5 ex |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11525129-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2788608-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2609646-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8512714-B2 |
priorityDate |
2004-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |