Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-36 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6462 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21073 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-49 |
filingDate |
2003-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2005-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcf6a8e517978ae1818ad3b47ec88660 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e3d8d959ae66514ab8b8a3f9ebdb186 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cda9bea460b69c75ac0110096c5feab3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aaf681c642ad09e00c6703d3e536b5e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4eac82320b96d712fdfaaca3931de5cd |
publicationDate |
2005-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
RU-2259212-C2 |
titleOfInvention |
Method for preparing active recombinant proteins |
abstract |
FIELD: molecular biology, medicinal industry. n SUBSTANCE: invention relates to a method for preparing recombinant double-stranded enzyme urokinase (ds-uAP). Method involves culturing cells CHO-Meissi subjected for genetic manipulations that are transfected stably with cDNA encoding pre-prourokinase in culturing medium containing alkanoic acids or their derivatives at temperature from 30°C to 37°C and the following separation the prepared ds-uAP for low-molecular and high-molecular forms. Also, invention involves recombinant ds-uAP, high-molecular ds-uAP and low-molecular ds-uAP in different stages of their preparing. Advantage of proposed method involves the development of method for preparing recombinant forms of mature double-stranded protein ds-uAP and its low-molecular and high-molecular forms. n EFFECT: improved preparing method. n 19 cl, 4 tbl, 5 ex |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2510820-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2491347-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2478702-C2 |
priorityDate |
2001-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |