http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2252782-C2
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-10 |
| filingDate | 2002-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2005-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54b41d4b3b87825b2c428b85420c2996 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3582aa8aac86c0c9f95f3bc3521bbec1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5431aaed1bd295a6c1b7a05affc79b0d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ad62402f5ab796524537fdd61653827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e9c5ae5e3ec8f1bff3088ac318321c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_833eaea04d0b0e01c7d5154c151cc1f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ec11df16e7c71b299d4f84b2206d455 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f01c6eef196f46f705e81e3ce6a911d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7cb643bdb6c2a6fb51dda38df345a10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e415c3d439d668695dcad36c6a7a4e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c138f0181659042b7db1c1d5df003047 |
| publicationDate | 2005-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2252782-C2 |
| titleOfInvention | Method for production of insulin drug of durable action |
| abstract | FIELD: pharmaceutical industry, in particular high quality insulin drug of durable action. n SUBSTANCE: claimed method includes providing of human insulin ester by transpeptization of porcine insulin at molar excess of threonine di-tert-butyl ester in aqueous/organic medium in presence of trypsin; acidifying for reaction inhibiting; chromatography purifying of obtained human insulin; deblocking of protective groups with trifluotoacetic acid and purifying of obtained crude human insulin; with subsequent dissolution in diluted acid, addition of acid solutions of zinc ions and protamine sulfate, mixing with buffered solution of m-cresol, phenol and glycerol; and solution conditioning to form crystals. Weight ratio of trypsin and porcine insulin is 1:300-1000. Before acidifying of reaction media reaction is additionally inhibited by mixture dilution with water by 2-3 times. Human insulin ester is purified using HPLC followed by deposition of ester derivatives fractions in presence of zinc ions and protective group deblocking to produce crystals of crude human insulin, which is purified again by HPLC, wherein both purifying processes are carried out using sorbent DIASOGEL ODS (C18) with particle size of 15 mum and pore size of 120 A as stationary phase, and as mobile phase in the first step 0.06 M-glycine HCl buffer, containing 0.015 M of ammonium sulfate and 20-30 % of propan-2-ol, having pH 2.5 is used, and in the second one 0.05 M acetate buffer containing 10-20 % of propan-2-ol with pH 2.5 is used. Then purified human insulin crystals are step-by-step dissolved in diluted acid to obtain fine dispersed suspension of insulin crystals in water; then diluted acid is added thereto, and mixture obtained after blending with buffered solution of cresol, phenol and glycerol, is held at 18-21°C for 20-22 h. n EFFECT: effective and economical method for insulin production with increased yield and purity; insulin of durable action and low immunological properties. n 12 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2472492-C2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9688737-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10259856-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9481721-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10265385-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10596231-B2 |
| priorityDate | 2001-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 160.