| titleOfInvention |
Vβ-Dβ-Jβ OLIGONUCLEOTIDE OF T-CELL RECEPTOR, PAIR PRIMERS, OLIGONUCLEOTIDE PROBE, METHOD FOR DETECTION OF MBP83-99Vβ13.1 CLONE PF T-CELLS, ESPRESSING LGRAGLTY MOTIVE OF T-CELL RECEPTOR, KIT, METHOD FOR TREATMENT OF AUTOIMMUNE DECEASE AND METHOD FOR MONITORING THEREOF |
| abstract |
FIELD: genetic engineering, medicine. n SUBSTANCE: invention relates to T-cell receptor sequence being detected in patients with extended sclerosis and is useful in diagnosis and therapy. Oligonucleotide including sequence which represents or is derived from 5'-CTAGGGCGGGCGGGACTCACCTAC-3' or nucleotide sequence being fully complementary thereto. Oligonucleotide together with nuclear acid including nearly 15-30 oligonucleotides, which doesn't comprise oligonucleotide sequence and presents in region from Vβ to Jβ of Vβ13.1 gene in T-cell Vβ13.1-subgroup, wherein oligonucleotide and nuclear acid sequences don't present in the same chain of pair sequences of Vβ13.1 gene, is used in Vβ13.1 gene part amplification. In method for detection of LGRAGLTY motive, which is present in T-cell receptors of T-cell Vβ13.1-subgroup, oligonucleotide is used in combination with labeling particle. Once LGRAGLTY motive is detected, development monitoring and treatment are carried out by removing of LGRAGLTY motive-containing peptide. n EFFECT: simplified methods for detection of LGRAGLTY motive in T-cell receptors and treatment of patients with extended sclerosis. n 21 cl, 7 dwg, 3 tbl, 3 ex |