http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2209830-C2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_562c70ebb17a141bda8f8bd27ce42d46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4ba7f369fbb6c3ebc792c495fe406f64 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
filingDate | 2001-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2003-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d459b84a3e186a1a05babf0ff90b6d7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8f3aa95f222c6a17fdb49e8037ce3d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2018228439e7765b5e976ddfa2409573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7c8f6bd10e24a9b2510711ba62afcd5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f06452701ecca3eb214b04ecd80f77c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21e0c2f6d1d69568039447bfb6f705f7 |
publicationDate | 2003-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2209830-C2 |
titleOfInvention | Set of oligonucleotides-primers for identification of hemorrhagic fever crimean-congo virus rna |
abstract | FIELD: molecular biology, biotechnology. SUBSTANCE: invention proposes a set of oligonucleotides-primers for identification of hemorrhagic fever Crimean-Congo virus RNA comprising 2 pairs of deoxyoligonucleotides eliciting activity of direct and reverse primers in reverse transcription reaction and polymerase chain reaction of the following structure: 5'-AGAGACTGAGGGGAAGGGAG-3' - PS1; 5'- AATTGTCTTGGCACACGGAT-3; - PS2; 5'-CAAAAGACT- GTAGAGGCTC-3' - PS3; 5'-TTGTCCGTAGGTTAAGAATG-3' - PS4 and also their analogues with degenerated structure: Ps1* 5'-AGAGAC(T,C)GAGGGGAA(A,G)GGAG-3'; Ps2* 5'-AATTGTCTTGGC- (A,G)CA(T,C)GGAT-3'; Ps3* 5'-CAA(A,G)AAGAC(T,C)GT(A, G)- GAGGCTC-3'; Ps4* 5'-TTGTCCG(T,A)AGGTT(A,G)AG(A,G)ATG-3' and direct control primer of the following structure: Ps5 5'-CT(T,C)- TCAGCTC(A,G)T(T, A)CTACTGGCT-3'. The use of primers set allows the detection of hemorrhagic fever Crimean-Congo virus taking into account variations of genome structure of different geographic isolates distributing in areas of Middle Asia and South regions of Russia. Invention can be used in medicine for detection of genetic material of hemorrhagic fever Crimean-Congo virus in samples. EFFECT: improved method of virus detection. 2 dwg, 1 tbl, 6 ex |
priorityDate | 2001-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
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