http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2206609-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4e06cbdb5469ae4df14e9162dd9856b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1998-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2003-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26ffb58c1237fe55ccf7345007e20ae8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ddc764f3a761ea7faf990339b92570c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba8dbc6e9505c3e903a7091fecce5d60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f77c8a1b64c3b8150f00827642d68b5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a429cd7c8fb543365d7f6601c19e871b |
| publicationDate | 2003-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2206609-C2 |
| titleOfInvention | Method for preparing recombinant dna molecule from one or more single-stranded or double-stranded matrix polynucleotides (variants) |
| abstract | FIELD: biotechnology, genetic engineering. SUBSTANCE: method of mutagenesis and recombination of polynucleotide sequences in vitro is based on elongation of primer oligonucleotides catalyzed by polymerase. Method involves priming matrix polynucleotide (polynucleotides) with primers with random sequences or well- defined sequences to provide pool of short DNA fragments with low level of point mutations. DNA fragments are subjected for denaturation followed by annealing and the following DNA polymerization catalyzed by enzyme. Procedure is repeated sufficiently times to obtain full-length genes that comprise mutants of the parent matrix polynucleotides. Then genes amplified using polymerase chain reaction and cloned into vector for expression of encoded proteins. Invention provides preparing gene products with improved properties. Invention can be used for realization of mutagenesis and recombination of polynucleotides sequences in vitro. EFFECT: improved preparing method. 62 cl, 17 dwg, 5 tbl |
| priorityDate | 1998-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.