http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2202616-C2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9d7ced77855b21f4260ba76e601ab720 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-82 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
| filingDate | 1992-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2003-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ec614fda2e38523688360f358f717d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54feec86b1ca491484c88ea511c7905f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7553889686dffc86f24dcda64d8dce00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4a76b84ea66873458d9125793b89913 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67aa122870e4e870e8f0ba6e2af66433 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75f718831f07080d99b51295b9b4a4b2 |
| publicationDate | 2003-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2202616-C2 |
| titleOfInvention | Method for preparing 7-aminocephalosporanic acid, expression vector, recombinant strain |
| abstract | FIELD: bio- technology, biochemistry, molecular biology, antibiotics. SUBSTANCE: invention relates to method for preparing vector based on technology of recombinant DNA. Vector is able to be inserted into chromosomal DNA of the strain Penicillium chrysogenum and provides expression DNA sequences from Cephalosporium acremonium encoding enzymes expandase/hydrolase and acetyltransferase under control of promoter IPNS Penicillium. As result of transformation with indicated vector of the strain Penicillium chrysogenum able to produce isopenicillin N recombinant strain is obtained where biosynthesis of all enzymes catalyzing the conversion of adipate to adipoyl-7-amino-cephalosporanic acid occurs. The example of such strain is Penicillium chrysogenum/pTS-8 (ATCC 74187). Adipoyl-7-aminocephalosporanic acid preparing by culturing the recombinant strain in the presence of adipate is treated then with adipoylamidase to form 7- aminocephalo-sporanic acid. Invention provides the possibility to prepare 7-amino-cephalosporanic acid in industrial scale by effective and economy method. Invention can be used in industrial preparing intermediate com- pounds used in synthesis of cephalosporin antibiotics. EFFECT: improved preparing method, enhanced effectiveness. 4 cl, 18 ex |
| priorityDate | 1992-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 392.