http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2197526-C1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_02ea32eac61873f4198f998db8915eff http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ebd222751dd7c79b02d72b4dbddf2afe http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_11762011fdb241a356d85f83ac477e71 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 |
filingDate | 2001-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2003-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f60ea0618c6bc11ce51e816651c2ac7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d331679d2d08bc958ce8dba1d559359e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35ed34dee5e9f9e7764f45929d83056c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d1dd7cc35d14c1eeeca39498aec1723 |
publicationDate | 2003-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | RU-2197526-C1 |
titleOfInvention | Dna pcx-302 fragment encoding synthesis of penicillium canescens secreted endo-(1-4)-beta-xylanase and fungus strain penicillium canescens as producer of secreted endo-(1-4)-beta-xylanase |
abstract | FIELD: genetic engineering, microbiology, biochemistry, enzymology. SUBSTANCE: invention relates to preparing DNA (PCX-302) fragment encoding synthesis of secreted endo-(1-4)-beta-xylanase based on isolation of genes of Penicillium canescens VKPM F-178 from phage library. Based on isolated DNA fragment the recombinant plasmid pPCXYLA is constructed and used for transformation of strain- -recipient of Penicillium canescens PCA 10 (nia D - ). As result of screening of transformants the multicopy strain P. canescens XYL-23 (VKPM F-832) as producer of enzyme secreted form is obtained. In culturing obtained strain in pectin-containing medium with beet pulp it is possible to obtain up to 770 U activity of endo-(1-4)- beta-xylanase per 1 ml of cultural medium that exceeds by 6-7-fold the enzyme yield in using the strain- recipient. Invention can be used for preparing enzyme endo-xylanase in industrial scale. EFFECT: increased yield of enzyme. 2 cl, 2 dwg |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9012186-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2711578-C1 |
priorityDate | 2001-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 201.