| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_489d43e5dc923e74f753f2e3bbc38e59 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24027 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-54 |
| filingDate |
1994-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2001-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2164daad86d97998d6c0ff8731285f75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8bbea86a94ead7137921faac2e1c4e8b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b9c771eed694af247a1bfb962c4edd4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1cd1c0655049a2d2d8906e3d8d5a66d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0529fe105053bcb95b7a508568f2c56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f17e58bda93155099e1b4b88383ae904 |
| publicationDate |
2001-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
RU-2167937-C2 |
| titleOfInvention |
Method of stabilizing aqueous solution or suspension of metalloprotease, and stabilized aqueous solution or suspension of metalloprotease |
| abstract |
FIELD: biochemical methods. SUBSTANCE: stabilization of enzyme is achieved by adding to its aqueous solution or suspension N-protected amino acid. When N-benzyloxycarbonylated amino acid is added to metalloprotease, the latter remains stable even in absence of excess of Ca ions. Enzyme can therefore be stored for a long time and also storage and transportation of liquid enzyme form are simplified. Regeneration of metalloprotease is improved in presence of N-protected amino acid when enzyme is used in reaction of binding between N-protected amino acid and amino acid ester. Most marked effect is observed in production of aspartame by reaction of benzyloxycarbonylaspartic acid with phenylalanine methyl ester. EFFECT: inhibited deactivation of metalloprotease and improved its stability. 8 cl, 6 tbl, 8 ex |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2484138-C2 |
| priorityDate |
1993-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |