http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2154822-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e6576e5f9d53e3a9cebfac145258ec9a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8dfb81fbcb188fd8d77f2654dd3be651 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate | 1999-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2000-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_657fd0c2a63ce97da9a34450a86faa43 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_574e548aed4402ef09f822656093a056 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79daae8e139c38eb0bf4404e575b2ca8 |
| publicationDate | 2000-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2154822-C1 |
| titleOfInvention | Method of isolation of bacterium of genus lactobacillus from clinical material |
| abstract | FIELD: medicinal microbiology. SUBSTANCE: method involves preparing a series of 10-fold dilutions of analyzed material in liquid selective nutrient enrichment medium at 39 C, seeding from dilutions on surface of solid selective nutrient medium, culturing seedings for 24-48 h at increased content of carbon dioxide gas (5%) and decreased oxygen concentration (16%) in culturing atmosphere. Liquid selective enrichment nutrient medium has the following composition, g/l: defatted milk liquid hydrolyzate, 225.0 ± 25.0; yeast concentrated autolyzate, 110.0 ± 10.0; agar, 0.8; distilled water, up to 1 l; 20% solution of sodium hydroxide, up to medium pH 5.4 ± 0.1. Then lactobacilli are isolated in liquid selective nutrient medium of the following composition, g/l: defatted milk liquid hydrolyzate, 225.0 ± 25.0; yeast concentrated autolyzate, 110.0 ± 10.0; agar, 22.5 ± 2.5; distilled water, up to 1 l; 20% solution of sodium hydroxide, up to medium pH 5.4 ± 0.1. Invention can be used for selective isolation of bacterium of genus Lactobacillus from clinical material (feces, vaginal secret). EFFECT: improved method of isolation, improved diagnosis of gynecological diseases. |
| priorityDate | 1999-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 178.