http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2146150-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4e8b2c9f74bd9bbe6ab2babd04bd2d9e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9976659971b0dd0e22b5402672a913bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_13e21bf3dec0ef60b9a7f0ac10610353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e023f8ef7cb02195590c3389075f06e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d360804508dac7ebc82ea942ca12def3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_174030fdfab770f7e2fb10cb6fe34a35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aa2e280a85d5884a5248e72e11b6a99c |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-559 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-21 |
| filingDate | 1999-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2000-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2704d997716ac540add753a7d5d18285 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c934abe9268408626e3a51f6dd935bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b9e0cef9b0c8981819bd934a50e7c86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02a520901f9630dcc0cc79af0b9d38e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88b3b39367d7c40c8cf7e370dd627036 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2ac8340a1da5f632d912a57eb0fb8ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64763d68eed5cc4c220298d56abb115b |
| publicationDate | 2000-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2146150-C1 |
| titleOfInvention | Method of preparing cultural antigen from equine infectious anemia virus and kit for antibody or antigen of equine infectious anemia virus |
| abstract | FIELD: biotechnology, veterinary virology. SUBSTANCE: method involves the use of the novel strain of equine infectious anemia virus N "3-K-VNIITIBP-VIEV" for antigen preparing. Strain is grown in horse embryo skin and/or muscle, or lung, and/or kidney cells up to accumulation of viruses at titer 10 5 ID 50/ml. Viruses are concentrated by ultrafiltration by 10-20-fold, lipid envelope is separated by treatment with ether in cold and the end product that is peptide (molecular mass is 24-26 kDa) carrying group-specific antigen determinants is obtained. After the concentration stage viral material can be subjected for purification by high-speed centrifugation and ultracentrifugation additionally. After separation of lipid envelope obtained antigen is purified by affinity chromatography additionally. In this case the end product is immunoelectrophoretically pure peptide. Obtained cultural antigen is used for kit making for identification of antigen of equine infectious anemia virus. Kits for identification of antibodies are designated for diagnosis of equine infectious anemia in RDP or IFA. Kit for identification of antigen is designated for titration of antigen of equine infectious anemia virus in the process of culturing. Kit for identification of antibodies in RDP has cultural antigen and specific precipitating serum. Kit for identification of antibodies in IFA has cultural antigen for immobilization on solid carrier, polystyrene plate and antispecies immunoglobulin class G (Ig G) conjugated with horse radish peroxidase. Kit for identification of antigen has cultural antigen for immobilization on solid carrier, polystyrene plate and Ig G to equine infectious anemia virus conjugated with horse radish peroxidase. Kits for IFA have gelatin, substrate for peroxidase reaction carrying out, detergent and buffer solutions also. Kits can contain positive and negative controls also. EFFECT: enhanced sensitivity, standard specification and specificity of cultural antigen and kits for identification of antibodies and antigen. 18 cl, 6 ex |
| priorityDate | 1999-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.