http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2144080-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b5196a9f24b47a0042672c5c946adbc |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-285 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-235 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H- |
| filingDate | 1988-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2000-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a0890b205aad573e773cdb4bfc15e2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2730f8547149077624ddb4bf34cdcc4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44852f75303826f426f28da6457cafee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3b3799698edb1f1e4fd4c089ac55e7d |
| publicationDate | 2000-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2144080-C1 |
| titleOfInvention | Ribosime, method of inactivation of rna-target, method of ribosime preparing |
| abstract | FIELD: molecular biology, biochemistry. SUBSTANCE: invention relates to synthetic single-stranded RNA molecules showing highly specific endonuclease activity. Ribosime has hybridizing and catalytic regions. Hybridizing region has one or more branches of a single-stranded RNA. The preferable ribosime has a catalytic region including the sequence AAGC*GAGXAGUC in "hairpin" N 1 * N 2 structure where * means bases pairing between complementary nucleotides, X means any ribonucleotide and N 1 and N 2 mean ribonucleotides with complementary sequences, at least, in part of their length. Ribosime splits RNA-target after triplet xUy where x is any ribonucleotide, U means uracil and y is adenine, cytosine or uracil. The nucleotide sequence encoding the required ribosime is obtained followed by its ligation with a suitable transfer vector containing DNA or RNA or their combination. The ligated sequence is transcribed using RNA-polymerase. RNA-target into the cells is subjected by ribosime effect that is able to carry out the specific cleavage of indicated RNA-target in the site taken so manner that the indicated RNA-target is hybridized into the cell with ribosime to induce inactivation of RNA-target. Invention ensures to obtain ribosimes that are able to hybridize effectively with the broad raw of target RNA-sequences but without modifying the cleavage RNA-targent. EFFECT: improved method of ribosime preparing. 11 cl, 38 dwg, 9 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2458986-C2 |
| priorityDate | 1987-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 442.