http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2133253-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_34cd435dab366d2351454495963fba5f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0078 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-715 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-02 |
| filingDate | 1994-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1999-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8692f8866aef4b4bca2e00da449998f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_432832fc3c294b4e775964bc50fa5567 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd010cb5de191470e1303c0b2e12ddb0 |
| publicationDate | 1999-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2133253-C1 |
| titleOfInvention | Purified heparin fraction, method of its preparing and pharmaceutical composition containing on said |
| abstract | FIELD: medicine, pharmacy. SUBSTANCE: invention relates to heparins of the low-molecular mass obtained by nitrose depolymerization of the natural heparin and containing nitros compounds at amount less or equal to 5 x 10 -7 mg/mg product. For removal of nitros compounds an aqueous solution of low-molecular mass heparin is treated by ultraviolet rays (wavelength is from 180 to 350 nm) at temperature 5-50 C and pH 3-8. For preparing the purified low-molecular mass heparin an aqueous solution of the natural heparin sodium salt (concentration is 5-15 wt. -%/vol. ) is treated with alkaline metal nitrite as measured 1.5-6.9 wt.-% nitrite to heparin salt taken. The indicated reaction is carried out in the presence of hydrochloric acid at pH from 1 to 5 for time from 20 to 50 min up to negative test for nitro-ions. Obtained product is reduced by addition of sodium boron hydride at amount 0.5-2.0 wt. -% of heparin salt amount taken. An excess of boron hydride is decomposed with hydrochloric acid. Obtained reaction medium is neutralized and the low-molecular mass heparin is precipitated with ethanol as a sodium salt. An aqueous solution of the obtained sodium salt is treated by ultraviolet rays. For preparing nitros compound-containing product at amount less or equal to 5 x 10 -8 mg/mg product the solution treated by ultraviolet rays is fed additionally on anion-exchange column. The indicated column is washed out with water at pH about 7 and the end product is recuperated by precipitation with sodium chloride and ethanol. Pharmaceutical composition based on the low-molecular mass purified heparin shows antithrombosis activity. Method of purification of the low-molecular mass heparin from nitros compounds by ultraviolet irradiation ensures to retain its biological and physical-chemical properties. EFFECT: improved method of preparing. 17 cl, 2 tbl, 2 dwg, 2 ex |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-186728-U1 |
| priorityDate | 1993-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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