| abstract |
FIELD: medicine, vaccines. SUBSTANCE: suspension of validated diploid cells of culture L-68 at concentration 200 000-300 000 cells/ml is infected with vaccine strain of measles virus. Multiple of infection is 0.1-0.01 TCD 50/cell. Infected cells are cultured at 32-35 C. After monolayer formation inert proteins of cultured medium are removed by 6-fold washing out with nutrient medium. Virus-containing liquid is collected at least once in every 24 h. Viral pourings at titer 5-6 lg TCD 50/0.5 ml and at protein content 16 mcg/ml, not above, are combined. Cellular detritus is removed by filtration, stabilized with sorbitol-gelatin and subjected for lyophilic drying. EFFECT: improved method of vaccine preparing. 3 cl, 3 tbl |