http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2109520-C1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_59766f3f624ec4d03bc3925b5d08d86a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d4302c6976b75c2b2848e910ba2f82fc |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-38 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-104 |
| filingDate | 1996-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1998-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f40f8027c9a4883cdb33c5c9135799b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ad03c7c13aeb8f4acb1a17e6a7302f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa81995de359d582f3df8bbc6513c67c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c4a57c5cc247da72f01d9dbef4568c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51353b98e73ec8f04b504477a400bea3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94608cd2e86e6101faf4fd5e62c90a47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ca39b025a840f8e2d84e16cae8c552e |
| publicationDate | 1998-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | RU-2109520-C1 |
| titleOfInvention | Method of preparing protective glycoprotein of melioidosis pathogen |
| abstract | FIELD: medicine, biochemistry. SUBSTANCE: invention relates to preparing glycoprotein antigen from B. pseudomallei. Method involves the use of two-day culture of virulent strain B. pseudomallei (Australian serovara) grown on cellophane film placed on solid nutrient medium surface. Culture is inactivated with acetone and an aqueous-salt extract is prepared by homogenization of 2% microbe cells suspension under mild conditions. Then homogeneity is subjected for successive fractionation by gel-filtration on carrier "Toyopearl HW-60", pH 7.2, and ion-exchange chromatography on DEAE-Sephadex A-50, pH 7.7, using 0.05 M tris-HCl as a buffer solution containing sodium chloride at concentration 0-1 M. Isolated glycoprotein consists of protein and carbohydrate at ratio 1:6 that shows homogeneity at immunoelectrophoresis assay. Significant protective effect is obtained after preliminary immunization of white mice with glycoprotein infected with virulent strains of Ps. pseudomallei. Preparation can be used for melioidosis vaccine making. EFFECT: improved method of preparing. 3 tbl |
| priorityDate | 1996-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.